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File:Chrom SephG-50.tif

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Summary

Description
English: Chromatogram showing the UV absorption profile of Arabidopsis cytosol separated on Sephadex G-50 Superfine. Gel volume: 500 mL; column length: 700 mm; column diameter: 30 mm; eluent flow rate: 12 mL / hr; fraction volume: 8.0 mL; number of fractions: 95; sample volume: 5 mL; separation temperature: 4 °C; elution buffer: 20 mM Tris-HCl, 1 mM NaN3; pH 8.0. Metal cofactors and associated proteins (MW ≥ 30 kDa) that eluted in the range of the void volume (120 to 140 mL) of this method were identified and quantified by ICP-MS or GF-AAS.
Date
Source Open Biochem J. 2008; 2: 44–48;doi: 10.2174/1874091X00802010044
Author Bernd Kastenholz
Permission
(Reusing this file)
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Captions

Chromatogram of cytosol of cadmium-contaminated Arabidopsis thaliana plants

Items portrayed in this file

depicts

inception

29 April 2008

media type

image/tiff

checksum

8c5f20dcdcf1b167429b120efd19b0987da8259c

determination method or standard: SHA-1

data size

79,642 byte

height

540 pixel

width

720 pixel

File history

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Date/TimeDimensionsUserComment
current08:08, 17 October 2010720 × 540 (78 KB)Indeblues~commonswiki{{Information |Description={{en|1=Chromatogram showing the UV absorption profile of Arabidopsis supernatant separated on Sephadex G-50 Superfine. Gel volume: 500 mL; column length: 700 mm; column diameter: 30 mm; eluent flow rate: 12 mL / hr; fraction vol

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