Meselson–Stahl experiment
The Meselson–Stahl experiment was an experiment done by Matthew Meselson and Franklin Stahl in 1958, using E. coli DNA. With their experiment, they found that DNA replication was semiconservative. The DNA consists of two helices that are combined. When the two helices are copied, each will have one part coming from the original cell. The other part will be newly made. In chemistry, that is called synthesis.
There are three different way this replication could happen:
- conservative replication: The original DNA is kept as it is. Two new helices will be synthesized from it.
- semi-conservative replication: Half of the DNA is kept, the other half is synthesized.
- disperse replication: The mechanism is the same as semi-conservative replication; but the synthesized links could be added to either the old or new helix.
Meselson and Stahl found that DNA employs semi-conservative replication. They put the bacteria in an environment with a Nitrogen isotope. They first used N15. The bacteria then integrated this isotope into their DNA. Later they used an environment that contained N14. They then looked at which of the isotopes the bacterial DNA contained. They found that it contained both isotopes of nitrogen, which implies that conservative replication is not the correct conclusion. By having a closer look they could then also rule out disperse replication. Some helix strands were all v. Other helix strands from the time in the N15 environment had N14.
Meselson–Stahl Experiment Media
Meselson-stahl experiment diagram en
References
- Meselson M. and Stahl F.W. (1958). "The replication of DNA in Escherichia coli". PNAS. 44 (7): 671–82. Bibcode:1958PNAS...44..671M. doi:10.1073/pnas.44.7.671. PMC 528642. PMID 16590258.