Sleeping Beauty transposon system

Sleeping Beauty (SB) transposon system is a non-viral vector for gene therapy (ref: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3095056/). The SB system enables high-level stable gene transfer and sustained transgene expression in multiple primary human somatic cell types, thereby representing a highly attractive gene transfer strategy for clinical use. (https://www.ncbi.nlm.nih.gov/pubmed/28964527). Sleeping Beauty (SB) transposon-based transfection is a two-component system consisting of a transposase and a transposon containing inverted repeat/direct repeat (IR/DR) sequences that result in precise integration into a TA dinucleotide. The transposon is designed with an expression cassette of interest flanked by IR/DRs, and SB transposase mediates stable integration and reliable long-term expression of the gene of interest. It has recently been demonstrated that SB efficiently mediates gene transfer and stable gene expression in human embryonic stem (ES) cells (http://cshprotocols.cshlp.org/content/2009/8/pdb.prot5270.full).