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File:Widespread-Expression-of-BORISCTCFL-in-Normal-and-Cancer-Cells-pone.0022399.s011.ogv

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English: Nucleus from a colorectal cell line, C99, stained with BORIS in green (Alexa 488) and fibrillarin in red (Alexa 594), with 4′, 6-Diamidino-2-phenylindole (DAPI) counterstaining in blue. BORIS immunoreactivity is enriched in the nucleolus adjacent to fibrillarin. Optical sections were obtained by confocal microscopy using a Zeiss Meta 510 LSM and three-dimensional reconstruction performed using Imaris x64 (v7.1.1). Surface reconstruction of each channel was performed with colour properties and transparency adjusted to show spatial distribution of BORIS with respect to fibrillarin in the nucleolus. Images are shown at 63x magnification.
Date
Source Movie S4 from Jones T, Ogunkolade B, Szary J, Aarum J, Mumin M, Patel S, Pieri C, Sheer D (2011). "Widespread Expression of BORIS/CTCFL in Normal and Cancer Cells". PLOS ONE. DOI:10.1371/journal.pone.0022399. PMID 21811597. PMC: 3139640.
Author Jones T, Ogunkolade B, Szary J, Aarum J, Mumin M, Patel S, Pieri C, Sheer D
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current20:36, 14 November 201220 s, 1,568 × 788 (5.61 MB)Open Access Media Importer BotAutomatically uploaded media file from Open Access source. Please report problems or suggestions here.

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