Knockout mouse
A knockout mouse is a genetically engineered mouse in which one or more genes have been turned off by a gene knockout.
Knockout mice are important animal models for studying the role of genes which have been sequenced but whose functions are not yet known. By causing a specific gene to be inactive in the mouse, and observing any differences from normal behaviour or condition, researchers can infer its probable function.
Mice are currently the most closely related laboratory animal species to humans for which the knockout technique can easily be applied. They are widely used in knockout experiments, especially those genetic questions which relate to human physiology.
Gene knockout in rats is much harder and has only been possible since 2003.[1][2]
The first knockout mouse was created by Mario R. Capecchi, Martin Evans and Oliver Smithies in 1989, for which they were awarded the Nobel Prize in Physiology or Medicine for 2007.
A detailed explanation of how knockout (KO) mice are produced is at the website of the Nobel Prize in Physiology or Medicine 2007.[3]
Aspects of the technology for generating knockout mice, and the mice themselves have been patented in many countries by private companies.
Knockout Mouse Media
A laboratory mouse in which a gene affecting hair growth has been knocked out (left) is shown next to a normal lab mouse.
A knockout mouse (left) that is a model for obesity, compared with a normal mouse
The procedure for making mixed-genotype blastocyst
Breeding scheme for producing knockout mice. Blastocysts containing cells, that are both wildtype and knockout cells, are injected into the uterus of a foster mother.
References
- ↑ naturenews, 19 May 2003
- ↑ "Y Zan et al., Production of knockout rats using ENU mutagenesis and a yeast-based screening assay, Nat. Biotechnol. (2003)" (PDF). Archived from the original (PDF) on 2010-06-11. Retrieved 2011-02-24.
- ↑ Nobel Prize detailed explanation of the work [1]