Nucleosome
Nucleosomes are the building blocks which make up chromosomes. They consist of a segment of DNA wound around a histone protein core.[1]
First, DNA and a protein make up chromatin. Then the chromatin is packed up into nucleosomes. Nucleosomes are then packaged up to make a chromosome. The packages do three things: the chromosomes function in cell division; the very long DNA is wrapped up in a shorter package; and the proteins regulate the gene action in cells.
Details
Nucleosomes form the basic repeating units of eukaryotic chromatin.[2] This packs the large eukaryotic genomes into the nucleus and allows it to be controlled.
In mammalian cells about two metres of linear DNA have to be packed into a nucleus of roughly 10 µm diameter. Nucleosomes are folded, in a series of higher order structures, to form a chromosome. This folding compacts the DNA and adds a layer of regulatory control. The control ensures correct gene expression.
Nucleosomes are thought to carry epigenetically inherited information, as modifications of their core histones. This information can be passed on to daughter cells, but it usually wiped out by meiosis in germ cells.
The nucleosome hypothesis proposed by Don and Ada Olins[3] and Roger Kornberg[4][5] in 1974, was a big step for understanding eukaryotic gene expression. Kornberg won the Nobel Prize in Chemistry (2006) for this and other discoveries.
Nucleosome dynamics
Although the nucleosome is a very stable protein-DNA complex, it is not static. It undergoes some structural re-arrangements, such as nucleosome sliding and DNA site exposure. Nucleosomes can inhibit or facilitate transcription.[6]
Since they were discovered in the mid 1960’s, histone changes were thought to affect transcription.[7]
Some modifications have been shown to be correlated with gene silencing, others seem to be correlated with gene activation. The information stored in this way is considered epigenetic: it is not coded in the DNA but is nevertheless inherited by daughter cells. The maintenance of a particular state of a gene is often necessary for cellular differentiation.[8]
Nucleosome Media
Basic units of chromatin structure
The crystal structure of the nucleosome core particle consisting of H2A , H2B , H3 and H4 core histones, and DNA. The view is from the top through the superhelical axis.
Apoptotic DNA laddering. Digested chromatin is in the first lane; the second contains DNA standard to compare lengths.
Scheme of nucleosome organization.
The crystal structure of the nucleosome core particle (Template:Pdb)
The current chromatin compaction model.
Histone tails and their function in chromatin formation
Diagram of nucleosome assembly.
Steps in nucleosome assembly
Nucleosome core particle 1EQZ v
References
- ↑
Reece, Jane; Campbell, Neil (2006). Biology. San Francisco: Benjamin Cummings. ISBN 0-8053-6624-5.
{{cite book}}: CS1 maint: multiple names: authors list (link) - ↑ Alberts, Bruce (2002). Molecular biology of the cell (4th ed.). New York: Garland Science. p. 207. ISBN 0-8153-4072-9.
- ↑ Olins A.L. & D.E. (1974). "Spheroid chromatin units (v bodies)". Science. 183 (4122): 330–2. Bibcode:1974Sci...183..330O. doi:10.1126/science.183.4122.330. PMID 4128918. S2CID 83480762.
- ↑ McDonald D. 2005. Milestone 9 (1973-1974) The nucleosome hypothesis: an alternative string theory. Nature Milestones: Gene expression. [1]
- ↑ Kornberg R.D. (May 1974). "Chromatin structure: a repeating unit of histones and DNA". Science. 184 (139): 868–71. Bibcode:1974Sci...184..868K. doi:10.1126/science.184.4139.868. PMID 4825889.
- ↑ Teif V.B. & Rippe K (2009). "Predicting nucleosome positions on the DNA: combining intrinsic sequence preferences and remodeler activities". Nucleic Acids Res. 37 (17): 5641–5655. doi:10.1093/nar/gkp610. PMC 2761276. PMID 19625488.
- ↑ Allfrey VG, Faulkner R, Mirsky A.E. (May 1964). "Acetylation and methylation of histones and their possible role in the regulation of RNA synthesis". Proc. Natl. Acad. Sci. U.S.A. 51 (5): 786–94. Bibcode:1964PNAS...51..786A. doi:10.1073/pnas.51.5.786. PMC 300163. PMID 14172992.
{{cite journal}}: CS1 maint: multiple names: authors list (link) - ↑ Felsenfeld G & Groudine M (2003). "Controlling the double helix". Nature. 421 (6921): 448–53. Bibcode:2003Natur.421..448F. doi:10.1038/nature01411. PMID 12540921. S2CID 681821.