Thiobacillus
Thiobacillus is a genus of bacteria which obtain energy through oxidisation of reduced sulfur compounds. This is sulfur with extra electrons. Thiobacillus species (spp.) exist in any environment with a source of reduced sulfur such as soils, hydrothermal vents, and sludge disposal sites [1]. Thiobacillus spp. grow best in temperatures from 28-32 °C, and pHs 2-8 [2].
To identify Thiobacillus spp., they can be viewed under a microscope. First, they need to be coloured with a gram stain. After staining, Thiobacillus spp. appear rod-shaped, and pink [1]. Pink means gram-negative and indicates no outer cell wall.
Classification
As of date, there are three established species of Thiobacillus genus: T. denitrificans, T. thioparus, T. thiophilus, and two candidates: T. plumbophilus, T. sajanensis[3]. These candidate species need to have their research papers verified. Historically, many species were classified under this genus. However, after the development of a new RNA sequencing technology known as “16S ribosomal RNA sequencing”, most were reclassified into new or existing genera. Confusingly, the older names of these reclassified bacteria are still used in the literature [4]. For example, Acidithiobacillus ferooxidans is sometimes referred to as Thiobacillus ferooxidans. However, they are no longer part of the Thiobacillus genus.
Many of the former members were mixotrophs. However, the remaining three species are completely reliant on carbon fixation, meaning the entire Thiobacillus genus can now be described as obligate autotrophs.
Sulfur oxidisation
Thiobacillus spp. get their food by using the carbon from CO2 and turning it into other compounds [5]. All Thiobacillus spp. use energy from sulfur oxidisation to provide themselves with energy.
Sulfur oxidisation is a reaction involving reduced sulfur and an electron acceptor - either oxygen or nitrate [6]. This process has two important outcomes: (1) the extra electrons in the reduced sulfur are freed, releasing energy, and (2) sulphate is produced[7]. An example source is the gas hydrogen sulfide (H2S).
Applications
Thiobacillus spp. are good for industrial use due to their sulfur oxidisation ability, low nutrient requirements, and their resilience to environmental extremes including high sulfide concentrations [7]. One application of Thiobacillus spp. is the removal of hydrogen sulfide. H2S is undesirable in biogas plants because they are: toxic, damage equipment, slow down biogas production, and contribute to the formation of sulfur dioxide (toxic gas)[7]. Thiobacillus spp. can oxidise the sulfide and turn it into a safer compound known as sulphate. This also reduces the level of sulfide. Similarly, Thiobacillus spp. is used in wastewater treatment. Wastewater contains sulfide, making it smelly and toxic [8]. Conversion to sulphate by Thiobacillus means the water can be safely deposited into the sea.
Another application is to produce sulphate in soil to help in agriculture. Sulfur is the fourth most essential plant nutrient [9]. However, reduced sulfur is not usable by plants. Sulphate is one of the two forms of sulfur that plants can take up. So, the bacterial oxidisation of sulfide to sulphate allows plants to take up sulfur through their roots [9]. To deal with low sulphate levels, it is possible to add sulphate directly to the soil. However, a longer-term solution is to promote the activity of Thiobacillus spp. Furthermore, Thiobacillus spp. can oxidise elemental sulfur. This produces sulfuric acid, which lowers the pH of the soil [8]. This is especially important in alkaline soils as it improves the ability of crops to take up other nutrients such as phosphorous, zinc, and iron [1].
References
- ↑ 1.0 1.1 1.2 Kumar, Murugan; Zeyad, Mohammad Tarique; Choudhary, Prassan; Paul, Surinder; Chakdar, Hillol; Singh Rajawat, Mahendra Vikram (2020), "Thiobacillus", Beneficial Microbes in Agro-Ecology, Elsevier, pp. 545–557, doi:10.1016/b978-0-12-823414-3.00026-5, ISBN 978-0-12-823414-3, retrieved 2024-07-15
- ↑ Almenglo, F.; González-Cortés, J.J.; Ramírez, M.; Cantero, D. (April 2023). "Recent advances in biological technologies for anoxic biogas desulfurization". Chemosphere. Elsevier. 321: 138084. doi:10.1016/j.chemosphere.2023.138084.
- ↑ Rana, Kavita; Rana, Neerja; Singh, Birbal (2020), "Applications of sulfur oxidizing bacteria", Physiological and Biotechnological Aspects of Extremophiles, Elsevier, pp. 131–136, doi:10.1016/b978-0-12-818322-9.00010-1, ISBN 978-0-12-818322-9, retrieved 2024-07-15
- ↑ Beheshti Ale Agha, Ali; Kahrizi, Danial; Ahmadvand, Asma; Bashiri, Hoda; Fakhri, Rosa (December 2018). "Identification of Thiobacillus bacteria in agricultural soil in Iran using the 16S rRNA gene". Molecular Biology Reports. 45 (6): 1723–1731. doi:10.1007/s11033-018-4316-3. ISSN 0301-4851.
- ↑ Robertson, Lesley A.; Kuenen, J. Gijs (2006), Dworkin, Martin; Falkow, Stanley; Rosenberg, Eugene; Schleifer, Karl-Heinz (eds.), "The Genus Thiobacillus", The Prokaryotes, New York, NY: Springer New York, pp. 812–827, doi:10.1007/0-387-30745-1_37, ISBN 978-0-387-25495-1, retrieved 2024-07-15
- ↑ Syed, M.; Soreanu, Gabriela; Falletta, Patricia; Beland, M. (January 2006). "Removal of hydrogen sulfide from gas streams using biological processes - A review". Canadian Biosystems Engineering 48. ResearchGate.
- ↑ 7.0 7.1 7.2 Wang, Ya-nan; Wang, Lei; Tsang, Yiu Fai; Fu, Xiaohua; Hu, Jiajun; Li, Huan; Le, Yiquan (September 2016). "The variability in carbon fixation characteristics of several typical chemoautotrophic bacteria at low and high concentrations of CO 2 and its mechanism". International Biodeterioration & Biodegradation. 113: 105–112. doi:10.1016/j.ibiod.2016.03.002.
- ↑ 8.0 8.1 "Sulfate and Elemental Sulfur – The Dynamic Duo". Sulfate and Elemental Sulfur – The Dynamic Duo. Retrieved 23 June 2024.
- ↑ 9.0 9.1 Lin, Sen; Mackey, Hamish R.; Hao, Tianwei; Guo, Gang; van Loosdrecht, Mark C.M.; Chen, Guanghao (October 2018). "Biological sulfur oxidation in wastewater treatment: A review of emerging opportunities". Water Research. 143: 399–415. doi:10.1016/j.watres.2018.06.051.