Vitamin C

ascorbic acid

Vitamin C, also known as ascorbic acid, is a vitamin. It is found in fresh fruits, berries and vegetables. It is one of the water-soluble vitamins.

Vitamin C is important in wound healing. Without enough vitamin C, a person can get a sickness called scurvy. Lack of vitamin C was a serious health problem on long ocean trips where supplies of fresh fruit were quickly used up. Many people died from scurvy on such trips.

Most animals make their own vitamin C. Some mammals cannot. Those that cannot include the main suborder of primates, the Haplorrhini: these are the tarsiers, monkeys and apes, including humans. Others are bats, capybaras and guinea pigs.

Vitamin C was first discovered in 1928. In 1932, it was proved to stop the sickness called scurvy. That fruit was a cure for scurvy was known long before vitamins were known to exist.

Potato with peel contains 20 mg/100 g of vitamin C

History

Through history the need for people to eat fresh plant food to help them get through long sieges or long sea trips was known by some wise people but was often forgotten.

The first attempt to prove this idea was by a ship's doctor in the Royal Navy called James Lind. At sea in May 1747 he gave some crew members lemon juice as well as their normal food, while others continued on normal food alone. The results showed that lemons prevented the disease. Lind wrote up his work and published it in 1753.

Lind's work was slow to be noticed. In 1795 the British navy adopted lemon or lime juice as food for sailors. The men hated the sour taste, and had to be forced to take it.

Limes, oranges; sauerkraut, salted cabbage, malt, and soup were tried with various effects. James Cook relied on sauerkraut to prevent the disease on his long voyages of exploration.

It was originally thought that only humans got scurvy. However, in 1907 Alex Holst and Theodore Frohlich, two Norwegian chemists found that guinea pigs could also get it if not given fresh food.

In 1928 the Arctic explorer Vilhjalmur Stefansson proved that Eskimos (Inuit) are able to avoid scurvy with almost no plant food in their diet by eating raw meat.

In 1912 the Polish American scientist Casimir Funk first used the word vitamin for something present in food in small amounts that is essential to health. He named the unknown thing that prevented scurvy Vitamin C.

From 1928 to 1933, the Hungarian research team of Joseph L Svirbely and Albert Szent-Gyorgyi, and separately the American Charles Glen King, first took out vitamin C from food and showed it to be an acid they called ascorbic acid.

In 1933/1934, the British chemists Norman Haworth and Edmund Hirst, and separately the Polish Tadeus Reichstein, successfully synthesized the vitamin. It was the first man-made vitamin. This made it possible to make lots of vitamin C cheaply in factories. Haworth won the 1937 Nobel Prize for Chemistry for this work.

In 1959 the American J.J. Burns showed that the reason why some animals get scurvy is because their liver cannot make one chemical enzyme that other animals have.

Vitamin C Media

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  Vitamin C supplements at a drug store.

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  The Nobel prizewinner Linus Pauling advocated taking vitamin C for the common cold in a 1970 book.

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  Vitamin C biosynthesis in plants

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  Citrus fruits were among the first sources of vitamin C available to ships' surgeons.

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  James Lind, a British Royal Navy surgeon who, in 1747, identified that a quality in fruit prevented scurvy in one of the first recorded controlled experiments.

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  Albert Szent-Györgyi wrote that he won a Nobel Prize after he found a way to mass-produce vitamin C for research purposes when he lived in Szeged, which had become the center of the paprika (red pepper) industry.

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  Albert Szent-Györgyi, pictured here in 1948, was awarded the 1937 Nobel Prize in Medicine "for his discoveries in connection with the biological combustion processes, with special reference to vitamin C and the catalysis of fumaric acid".

Plant sources

Citrus fruits (such as lime, Indian gooseberry, lemon, orange, and grapefruit) are good sources of vitamin C.

Other foods that are good sources of vitamin C include papaya, broccoli, brussels sprouts, blackcurrants, strawberries, cauliflower, spinach, cantaloupe, sweet peppers, and kiwifruit.

The following table is to give an idea of how much vitamin C is in different plant foods. Each individual fruit will vary.

The amount of vitamin C in foods of plant origin depends on the kind of plant, the kind of soil where it grew, how much rain and sun it got, the length of time since it was picked, and how it was stored since then. Cooking food destroys vitamin C.

Table Showing Relative Abundance of Vitamin C in Principal Fruits and some Raw Vegetables

Fruit	mg vitamin C per 100 grams of fruit	Fruit Continued	mg vitamin C per 100 grams of fruit	Fruit Continued	mg vitamin C per 100 grams of fruit
CamuCamu	2800	Lemon	40	Grape	10
Rose hip	2000	Melon, cantaloupe	40	Apricot	10
Acerola	1600	Cauliflower	40	Plum	10
Jujube	500	Grapefruit	30	Watermelon	10
Baobab	400	Raspberry	30	Banana	9
Blackcurrant	200	Tangerine/ Mandarin oranges	30	Carrot	9
Indian gooseberry	445	Passion fruit	30	Avocado	8
Guava	100
Kiwifruit	90	Spinach	30	Crabapple	8
Broccoli (raw)	90	Cabbage (raw green)	30	Peach	7
Loganberry	80	Lime	20	Apple	6
Redcurrant	80	Mango	20	Blackberry	6
Brussels sprouts	80	Melon, honeydew	20	Beetroot	5
Lychee	70	Raspberry	20	Pear	4
Persimmon	60	Tomato	10	Lettuce	4
Papaya	60	Blueberry	10	Cucumber	3
Strawberry	50	Pineapple	10	Fig	2
Orange	50	Pawpaw	10	Bilberry	1

Animal sources

Most species of animals synthesise their own vitamin C. It is therefore not a vitamin for them. It is made by a series of enzyme-driven steps which convert glucose to ascorbic acid. This is done either in the kidneys (reptiles and some birds), or the liver (mammals and perching birds). The loss of an enzyme which is essential for ascorbic acid synthesis has occurred quite frequently in evolution. It has happened in most fish, many birds; some bats, guinea pigs and most but not all primates, including humans. The mutations have not been lethal because ascorbic acid is so common in various food sources, especially fruit.

It was only realised in the 1920s that some cuts of meat and fish are also a source of vitamin C. However, the muscle and fat which make up the modern western diet are poor sources. As with fruit and vegetables cooking destroys the vitamin C content.

Table Showing Relative Abundance of Vitamin C in Foods of Animal Origin

Food of animal origin	mg vitamin C per 100 grams food	Food of animal origin (contd)	mg vitamin C per 100 grams food	Food of animal	mg vitamin C per 100 grams food
Calf liver (raw)	36	Chicken liver (fried )	13	Goats milk (fresh)	2
Beef liver (raw)	31	Lamb liver (Fried)	12	Beef steak (fried)	0
Oysters (raw)	30	Lamb heart (roast)	11	Hens egg (raw )	0
Cod Roe (fried)	26	Lamb tongue (stewed)	6	Pork Bacon (fried)	0
Pork liver (raw)	23	Human milk (fresh)	4	Calf veal cutlet (fried)	0
Lamb brain (boiled)	17	Cows milk (fresh)	2	Chicken leg (roast)	0

Artificial chemical synthesis

Vitamin C is produced from glucose by two main routes. The Reichstein process developed in the 1930s uses a single pre-fermentation followed by a purely chemical route. The more modern Two-Step fermentation process was originally developed in China in the 1960s, uses additional fermentation to replace part of the later chemical stages. Both processes yield approximately 60% vitamin C from the glucose feed.

In 1934, the Swiss pharmaceutical company Hoffmann-La Roche was the first to mass-produce synthetic vitamin C, under the brand name of Redoxon. Main producers today are BASF/ Takeda, Roche, Merck and the China Pharmaceutical Group Ltd of the People's Republic of China.

Functions of vitamin C in the body

In living organisms, ascorbate is an antioxidant, since it protects the body against oxidative stress. It is also a cofactor in at least eight enzymatic reactions, including several collagen synthesis reactions that cause the most severe symptoms of scurvy when they are dysfunctional. In animals, these reactions are especially important in wound-healing and in preventing bleeding from capillaries.

• Vitamin C is needed for the production of collagen in the connective tissue. These fibres are in many places throughout the body; providing firm but flexible structure. Some tissues have a greater percentage of collagen, especially: skin, mucous membranes, teeth, and bones.
• Vitamin C is required for making of dopamine, noradrenaline, and adrenaline in the nervous system or in the adrenal glands.
• Vitamin C is also needed to make carnitine, important in the transfer of energy to the cell mitochondria.
• It is a strong antioxidant.
• The tissues with greatest percentage of vitamin C—over 100 times the level in blood plasma—are the adrenal glands, pituitary, thymus, corpus luteum, and retina.
• The brain, spleen, lung, testicle, lymph nodes, liver, thyroid, small intestinal mucosa, leukocytes, pancreas, kidney, and salivary glands usually have 10 to 50 times the concentration present in plasma.

Vitamin C deficiency

Lack of ascorbic acid in the daily diet leads to a disease called scurvy, a form of avitaminosis that is characterized by:

• Loose teeth
• Superficial bleeding
• Fragility of blood vessels
• Poor healing
• Compromised immunity
• Mild anemia.

Daily requirement

A healthy person on a balanced western diet should be able to get all the vitamin C needed to prevent the symptoms of scurvy from their daily diet. People who smoke, those under stress and women in pregnancy have a slightly higher requirement.

The amount of vitamin C needed to avoid deficiency symptoms and maintain health has been set by variously national agencies as follows:

• 40 mg per day UK Food Standards Agency
• 60–95 mg per day US Food and Nutrition Board 2001 revision.

Some researchers have calculated the amount needed for an adult human to achieve similar blood serum levels as Vitamin C synthesising mammals as follows:

• 200 mg per day - Linus Pauling Institute and US National Institutes of Health (NIH) Recommendation.
• 3000 mg per day - Vitamin C Foundation's recommendation.
• 6000–12000 mg per day–Thomas Levy, Colorado Integrative Medical Centre recommendation.
• 6000–18000 mg per day - Linus Pauling's daily recommendation

High doses (thousands of mg) may result in diarrhoea, which is harmless if the dose is reduced immediately. Some researchers (Cathcart) claim the onset of diarrhoea to be an indication of where the body’s true vitamin C requirement lies.

The small size of the ascorbic acid molecule means the kidneys cannot retain it in the body. Quite a low level in the blood serum will cause traces to be present in the urine. All vitamin C synthesising mammals have traces in the urine at all times.

In April 1998 Nature reported alleged carcinogenic and teratogenic effects of excessive doses of vitamin C. This was given great prominence in the world's media. The effects were noted in test tube experiments and on only two of the 20 markers of free radical damage to DNA. They have not been supported by further evidence from living organisms. Almost all mammals manufacture their own vitamin C in amounts equivalent to human doses of thousands of milligrams per day. Large amounts of the vitamin are used in orthomolecular medicine and no harmful effects have been observed even in doses of 10,000 mg per day or more.

Therapeutic uses

Vitamin C is needed in the diet to prevent scurvy. It also has a reputation for being useful in the treatment of colds and flu. The evidence to support this idea, however, is ambiguous and the effect may depend on the dose size and dosing regime. The Vitamin C Foundation recommends 8 grams of vitamin C every half hour to show an effect on cold symptoms.

Vitamin C advocacy

Fred R. Klenner, a doctor in Reidsville, North Carolina reported in 1949 that poliomyelitis yielded to repeated megadoses of intravenous vitamin C.

Nobel Prize winning chemist Linus Pauling began actively promoting vitamin C in the 1960s as a means to greatly improve human health and resistance to disease.

A minority of medical and scientific opinion continues to see vitamin C as being a low cost and safe way to treat infectious disease and to deal with a wide range of poisons.

A meta-study of the published research claimed that relatively high levels of vitamin C must be maintained in the body for it to function effectively as an antioxidant.

Some research shows that there are veterinary benefits of vitamin C as well.

One meta-study of the published research examined the effectiveness of ascorbic acid in the treatment of infectious disease and toxins. It was conducted in 2002 by Dr. Thomas Levy, medical director of the Colorado Integrative Medical Center in Denver. It claimed that overwhelming scientific evidence exists for its therapeutic role.

Some vitamin C advocates say that vitamin C is not used therapeutically because it cannot be patented. Pharmaceutical companies seek to generate revenue and profit their shareholders. They may be reluctant to research or promote something that will make them little money.